カタログ製品コード : C-EK201BHS-96

Mouse IL-1β High Sensitivity ELISA kit

*写真はイメージです
24T ¥65,900 (¥2,746/T) (税別)
48T ¥82,600 (¥1,721/T) (税別)
96T ¥94,000 (¥979/T) (税別)
標準納期 : 2週間
メーカー名 遺伝子名 種交差性 測定範囲 サンプル量 適用サンプル ドキュメント
MultiSciences IL-1β Mouse 1.56 - 100 pg/ml 10 μl 血清、血漿、細胞培養上清

保存方法 :

キットは未開封の場合、4℃で保管すること。 開封後は標準溶液を-20℃で保存し、他の試薬は4℃で保存すること。

構成内容 :

96-well polystyrene microplate coated with a monoclonal antibody against IL-1b
Mouse IL-1b Standard, lyophilized
IL-1b Detect Antibody
Standard Diluent
Streptavidin-HRP
Amplification Reagent Concentrate
Amplification Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers

キーワード :

Mus musculus,Mouse,Interleukin-1 beta,IL-1 beta,Il1b

ターゲット情報 :

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-1β present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-1β is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.