カタログ製品コード : C-EK110HS-96
Human IL-10 High Sensitivity ELISA kit
| 24T | ¥61,900 | (¥2,579/T) (税別) |
| 48T | ¥78,600 | (¥1,638/T) (税別) |
| 96T | ¥90,000 | (¥938/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| MultiSciences | IL-10 | Human | 6.25 - 400 pg/ml | 10 μl | 血清、血漿、細胞培養上清 |
|
■保存方法 :
キットは未開封の場合、4℃で保管すること。 開封後は標準溶液を-20℃で保存し、他の試薬は4℃で保存すること。
■構成内容 :
| 96-well polystyrene microplate coated with a monoclonal antibody against IL-10 |
| Rat IL-10 Standard, lyophilized |
| IL-10 Detect Antibody |
| Standard Diluent |
| Streptavidin-HRP |
| Amplification Reagent Concentrate |
| Amplification Diluent |
| Assay Buffer (10×) |
| Substrate (TMB) |
| Stop Solution |
| washing Buffer (20×) |
| Plate Covers |
■キーワード :
Homo sapiens,Human,Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,IL10
■ターゲット情報 :
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for rat IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-10 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-10 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
