カタログ製品コード : C-E2298h
ELISA kit for Glycogen synthase kinase-3 beta
| 24T | ¥75,600 | (¥3,150/T) (税別) |
| 48T | ¥86,500 | (¥1,802/T) (税別) |
| 96T | ¥102,800 | (¥1,071/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| EIAab | GSK3B | Human | 0.312-20 ng/ml | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
|
■保存方法 :
一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度
長期保存の場合
－20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSK-3 beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GSK-3 beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GSK-3 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSK-3 beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20ml |
| Assay Diluent A | 1 × 10ml |
| Assay Diluent B | 1 × 10ml |
| Detection Reagent A | 1 × 120μl |
| Detection Reagent B | 1 × 120μl |
| Wash Buffer(25 x concentrate) | 1 × 30ml |
| Substrate | 1 × 10ml |
| Stop Solution | 1 × 10ml |
| Plate sealer for 96 wells | 5 |
| Instruction | 1 |
■キーワード :
Homo sapiens,Human,Glycogen synthase kinase-3 beta,GSK-3 beta,Serine/threonine-protein kinase GSK3B,GSK3B,2.7.11.26,2.7.11.1
■ターゲット情報 :
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.