カタログ製品コード : C-E2085h
ELISA kit for Angiopoietin-related protein 4
| 24T | ¥75,600 | (¥3,150/T) (税別) |
| 48T | ¥86,500 | (¥1,802/T) (税別) |
| 96T | ¥102,800 | (¥1,071/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| EIAab | ANGPTL4 | Human | 0.156-10 ng/mL | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
|
■保存方法 :
一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度
長期保存の場合
－20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20ml |
| Assay Diluent A | 1 × 10ml |
| Assay Diluent B | 1 × 10ml |
| Detection Reagent A | 1 × 120μl |
| Detection Reagent B | 1 × 120μl |
| Wash Buffer(25 x concentrate) | 1 × 30ml |
| Substrate | 1 × 10ml |
| Stop Solution | 1 × 10ml |
| Plate sealer for 96 wells | 5 |
| Instruction | 1 |
■キーワード :
Homo sapiens,Human,Angiopoietin-related protein 4,Angiopoietin-like protein 4,Hepatic fibrinogen/angiopoietin-related protein,HFARP,ANGPTL4,ARP4,HFARP,PGAR,PP1158,PSEC0166,
■ターゲット情報 :
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.