カタログ製品コード : C-E1388h
ELISA kit for Neutrophil gelatinase-associated lipocalin
| 24T | ¥75,600 | (¥3,150/T) (税別) |
| 48T | ¥86,500 | (¥1,802/T) (税別) |
| 96T | ¥102,800 | (¥1,071/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| EIAab | LCN2 | Human | 0.156-10 ng/mL | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
|
■保存方法 :
一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度
長期保存の場合
－20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NGAL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NGAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20ml |
| Assay Diluent A | 1 × 10ml |
| Assay Diluent B | 1 × 10ml |
| Detection Reagent A | 1 × 120μl |
| Detection Reagent B | 1 × 120μl |
| Wash Buffer(25 x concentrate) | 1 × 30ml |
| Substrate | 1 × 10ml |
| Stop Solution | 1 × 10ml |
| Plate sealer for 96 wells | 5 |
| Instruction | 1 |
■キーワード :
Homo sapiens,Human,Neutrophil gelatinase-associated lipocalin,NGAL,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Lipocalin-2,Oncogene 24p3,p25,LCN2,HNL,NGAL,p25
■ターゲット情報 :
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
