カタログ製品コード : C-E1180m

ELISA kit for Clusterin

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24T ¥78,500 (¥3,271/T) (税別)
48T ¥89,800 (¥1,871/T) (税別)
96T ¥106,800 (¥1,113/T) (税別)
標準納期 : 2週間
メーカー名 遺伝子名 種交差性 測定範囲 サンプル量 適用サンプル ドキュメント
EIAab Clu Mouse 1.56-100 ng/ml 100 μl 血清、血漿、尿、組織ホモジネート、細胞培養上清等

保存方法 :

一時保存の場合
TMB:4℃
試薬類:バイアルに記載の温度

長期保存の場合
-20℃

測定原理 :

The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

構成内容 :

ReagentQuantity
Assay plate1
Standard2
Sample Diluent1 × 20ml
Assay Diluent A1 × 10ml
Assay Diluent B1 × 10ml
Detection Reagent A1 × 120μl
Detection Reagent B1 × 120μl
Wash Buffer(25 x concentrate)1 × 30ml
Substrate1 × 10ml
Stop Solution1 × 10ml
Plate sealer for 96 wells5
Instruction1
 

キーワード :

Mus musculus,Mouse,Clusterin,Apolipoprotein J,Apo-J,Clustrin,Sulfated glycoprotein 2,SGP-2,Clu,Apoj,Msgp-2

ターゲット情報 :

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation.