カタログ製品コード : C-E0928h
ELISA kit for Cellular tumor antigen p53
| 24T | ¥89,900 | (¥3,746/T) (税別) |
| 48T | ¥103,100 | (¥2,148/T) (税別) |
| 96T | ¥122,800 | (¥1,279/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| EIAab | TP53 | Human | 0.156-10 ng/ml | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
|
■保存方法 :
一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度
長期保存の場合
－20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20ml |
| Assay Diluent A | 1 × 10ml |
| Assay Diluent B | 1 × 10ml |
| Detection Reagent A | 1 × 120μl |
| Detection Reagent B | 1 × 120μl |
| Wash Buffer(25 x concentrate) | 1 × 30ml |
| Substrate | 1 × 10ml |
| Stop Solution | 1 × 10ml |
| Plate sealer for 96 wells | 5 |
| Instruction | 1 |
■キーワード :
Homo sapiens,Human,Cellular tumor antigen p53,Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53,TP53,P53
■ターゲット情報 :
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.