■保存方法 :

一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度

長期保存の場合
－20℃

■測定原理 :

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HIF-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HIF-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIF-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

■構成内容 :

Reagent	Quantity
Assay plate	1
Standard	2
Sample Diluent	1 × 20ml
Assay Diluent A	1 × 10ml
Assay Diluent B	1 × 10ml
Detection Reagent A	1 × 120μl
Detection Reagent B	1 × 120μl
Wash Buffer(25 x concentrate)	1 × 30ml
Substrate	1 × 10ml
Stop Solution	1 × 10ml
Plate sealer for 96 wells	5
Instruction	1

■キーワード :

Rattus norvegicus,Rat,Hypoxia-inducible factor 1-alpha,HIF-1-alpha,HIF1-alpha,Hif1a