カタログ製品コード : C-E0528h
ELISA kit for Platelet-derived growth factor C
| 24T | ¥75,600 | (¥3,150/T) (税別) |
| 48T | ¥86,500 | (¥1,802/T) (税別) |
| 96T | ¥102,800 | (¥1,071/T) (税別) |
標準納期 : 2週間 |
製品詳細
| メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
|---|---|---|---|---|---|---|
| EIAab | PDGFC | Human | 78-5000 pg/mL | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
|
■保存方法 :
一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度
長期保存の場合
－20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDGF-C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDGF-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDGF-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20ml |
| Assay Diluent A | 1 × 10ml |
| Assay Diluent B | 1 × 10ml |
| Detection Reagent A | 1 × 120μl |
| Detection Reagent B | 1 × 120μl |
| Wash Buffer(25 x concentrate) | 1 × 30ml |
| Substrate | 1 × 10ml |
| Stop Solution | 1 × 10ml |
| Plate sealer for 96 wells | 5 |
| Instruction | 1 |
■キーワード :
Homo sapiens,Human,Platelet-derived growth factor C,PDGF-C,Fallotein,Spinal cord-derived growth factor,SCDGF,VEGF-E,PDGFC,SCDGF,UNQ174/PRO200
■ターゲット情報 :
Potent mitogen and chemoattractant for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. Acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding to receptors induces their activation by tyrosine phosphorylation. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Seems to be involved in palatogenesis.