■保存方法 :

一時保存の場合
TMB：4℃
試薬類：バイアルに記載の温度

長期保存の場合
－20℃

■測定原理 :

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4a, During the reaction, C4a in the sample or standard competes with a fixed amount of biotin-labeled C4a for sites on a pre-coated Monoclonal antibody specific to C4a. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C4a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

■構成内容 :

Reagent	Quantity
Assay plate	1
Standard	2
Sample Diluent	1 × 20ml
Assay Diluent A	1 × 10ml
Assay Diluent B	1 × 10ml
Detection Reagent A	1 × 60μl
Detection Reagent B	1 × 120μl
Wash Buffer(25 x concentrate)	1 × 30ml
Substrate	1 × 10ml
Stop Solution	1 × 10ml
Plate sealer for 96 wells	5
Instruction	1

■キーワード :

Triiodothyronine,T3,