カタログ製品コード : C-E0144b

ELISA kit for Vascular endothelial growth factor B

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24T ¥65,200 (¥2,717/T) (税別)
48T ¥74,300 (¥1,548/T) (税別)
96T ¥87,800 (¥915/T) (税別)
標準納期 : 2週間
メーカー名 遺伝子名 種交差性 測定範囲 サンプル量 適用サンプル ドキュメント
EIAab VEGFB Bovine 31.2-2000 pg/mL 100 μl 血清、血漿、尿、組織ホモジネート、細胞培養上清等

保存方法 :

一時保存の場合
TMB:4℃
試薬類:バイアルに記載の温度

長期保存の場合
-20℃

測定原理 :

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VEGF-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VEGF-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VEGF-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

構成内容 :

ReagentQuantity
Assay plate1
Standard2
Sample Diluent1 × 20ml
Assay Diluent A1 × 10ml
Assay Diluent B1 × 10ml
Detection Reagent A1 × 120μl
Detection Reagent B1 × 120μl
Wash Buffer(25 x concentrate)1 × 30ml
Substrate1 × 10ml
Stop Solution1 × 10ml
Plate sealer for 96 wells5
Instruction1
 

キーワード :

Bos taurus,Bovine,Vascular endothelial growth factor B,VEGF-B,VEGF-related factor,VRF,VEGFB,VRF

ターゲット情報 :

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.