カタログ製品コード : C-E0120h

ELISA kit for Kit ligand

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24T ¥75,600 (¥3,150/T) (税別)
48T ¥86,500 (¥1,802/T) (税別)
96T ¥102,800 (¥1,071/T) (税別)
標準納期 : 2週間
メーカー名 遺伝子名 種交差性 測定範囲 サンプル量 適用サンプル ドキュメント
EIAab KITLG Human 31.2-2000 pg/mL 100 μl 血清、血漿、尿、組織ホモジネート、細胞培養上清等

保存方法 :

一時保存の場合
TMB:4℃
試薬類:バイアルに記載の温度

長期保存の場合
-20℃

測定原理 :

The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

構成内容 :

ReagentQuantity
Assay plate1
Standard2
Sample Diluent1 × 20ml
Assay Diluent A1 × 10ml
Assay Diluent B1 × 10ml
Detection Reagent A1 × 120μl
Detection Reagent B1 × 120μl
Wash Buffer(25 x concentrate)1 × 30ml
Substrate1 × 10ml
Stop Solution1 × 10ml
Plate sealer for 96 wells5
Instruction1
 

キーワード :

Homo sapiens,Human,Kit ligand,Mast cell growth factor,MGF,Stem cell factor,SCF,c-Kit ligand,KITLG,MGF,SCF

ターゲット情報 :

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.