カタログ製品コード : C-E0048h
ELISA kit for Intercellular adhesion molecule 1
24T | ¥59,500 | (¥2,479/T) (税別) |
48T | ¥67,600 | (¥1,408/T) (税別) |
96T | ¥79,800 | (¥831/T) (税別) |
標準納期 : 2週間 |
カタログ製品コード : C-E0048h
ELISA kit for Intercellular adhesion molecule 1
24T | ¥59,500 | (¥2,479/T) (税別) |
48T | ¥67,600 | (¥1,408/T) (税別) |
96T | ¥79,800 | (¥831/T) (税別) |
標準納期 : 2週間 |
メーカー名 | 遺伝子名 | 種交差性 | 測定範囲 | サンプル量 | 適用サンプル | ドキュメント |
---|---|---|---|---|---|---|
EIAab | ICAM1 | Human | 31.2-2000 pg/mL | 100 μl | 血清、血漿、尿、組織ホモジネート、細胞培養上清等 |
■保存方法 :
一時保存の場合
TMB:4℃
試薬類:バイアルに記載の温度
長期保存の場合
-20℃
■測定原理 :
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICAM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICAM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
■構成内容 :
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20ml |
Assay Diluent A | 1 × 10ml |
Assay Diluent B | 1 × 10ml |
Detection Reagent A | 1 × 120μl |
Detection Reagent B | 1 × 120μl |
Wash Buffer(25 x concentrate) | 1 × 30ml |
Substrate | 1 × 10ml |
Stop Solution | 1 × 10ml |
Plate sealer for 96 wells | 5 |
Instruction | 1 |
■キーワード :
Homo sapiens,Human,Intercellular adhesion molecule 1,ICAM-1,Major group rhinovirus receptor,ICAM1,CD54
■ターゲット情報 :
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.