カタログ製品コード : C-E0048h

ELISA kit for Intercellular adhesion molecule 1

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24T ¥59,500 (¥2,479/T) (税別)
48T ¥67,600 (¥1,408/T) (税別)
96T ¥79,800 (¥831/T) (税別)
標準納期 : 2週間
メーカー名 遺伝子名 種交差性 測定範囲 サンプル量 適用サンプル ドキュメント
EIAab ICAM1 Human 31.2-2000 pg/mL 100 μl 血清、血漿、尿、組織ホモジネート、細胞培養上清等

保存方法 :

一時保存の場合
TMB:4℃
試薬類:バイアルに記載の温度

長期保存の場合
-20℃

測定原理 :

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICAM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICAM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICAM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

構成内容 :

ReagentQuantity
Assay plate1
Standard2
Sample Diluent1 × 20ml
Assay Diluent A1 × 10ml
Assay Diluent B1 × 10ml
Detection Reagent A1 × 120μl
Detection Reagent B1 × 120μl
Wash Buffer(25 x concentrate)1 × 30ml
Substrate1 × 10ml
Stop Solution1 × 10ml
Plate sealer for 96 wells5
Instruction1
 

キーワード :

Homo sapiens,Human,Intercellular adhesion molecule 1,ICAM-1,Major group rhinovirus receptor,ICAM1,CD54

ターゲット情報 :

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.